The Growth and Differentiation Potential of Dental Pulp Cells in Demineralized Dentin Tubules
Researchers from the Graduate School of Tokyo Dental College, Ziauddin University, Hayatabad Medical Complex, and King Saud University attempted to regenerate a biocompatible osteodentin-like, hard-tissue matrix in order to replace gutta-percha.
Researchers from the Graduate School of Tokyo Dental College, Ziauddin University, Hayatabad Medical Complex, and King Saud University attempted to regenerate a biocompatible osteodentin-like, hard-tissue matrix in order to replace gutta-percha. Their study has been published in Bosnian Journal of Basic Medical Sciences.
Using immunohistochemical analysis, they confirmed that dentin-like hard tissue was produced that was composed of dentin-forming cells, including odontoblasts and osteoblasts.
The team transplanted dental pulp cells embedded in collagen-type gel and loaded into demineralized dentin tubules. Thirty-two Sprague-Dawley rats were used in this experiment, while six green fluorescent protein-transgenic rats were used as donor for the collection of pulp cells.
After three weeks of the experiment, the researchers found osteodentin-like hard tissue inside the demineralized dentin tubules. Osteodentin-like hard tissue matrix was immunohistochemically analyzed with the bone and dentin antibodies like alkaline phosphatase, bone sialoprotein, osteopontin, nestin, and dentin sialoprotein.
The researchers used cultured or expanded pulp cells up to third passage and growth factor of bone morphogenetic protein expressed within the surface of the root canal with EDTA.
Read the press release here: https://sciencex.com/wire-news/358141686/the-growth-and-differentiation-potential-of-dental-pulp-cells-in.html