A peer-reviewed journal that offers evidence-based clinical information and continuing education for dentists.

Start earning CE Units in minutes!

Course Library New User Existing User Help

Conventional and Alternative Approaches to Enamel Remineralization

While fluoride is the gold standard in remineralization therapy, alternative approaches are available — including natural agents.

This course was published in the December 2022 issue and expires December 2025. The author has no commercial conflicts of interest to disclose. This 2 credit hour self-study activity is electronically mediated.

AGD Subject Code: 010


After reading this course, the participant should be able to:

  1. Explain the processes of demineralization and remineralization.
  2. Describe fluoride’s mechanisms of action in promoting
  3. Discuss fluoride alternatives that can be used when managing
    enamel demineralization.

Biomineralization is a lifelong, complex process by which the body produces minerals in order to strengthen existing hard structures.1 When the body’s pH homeostasis is disrupted, the result may be demineralization of hard tissue, such as tooth and bone, or a precipitation of minerals, such as occurs in the development of kidney stones or gallstones.2

In dentistry, the focus is on preventing demineralization of enamel to avoid the eventual development of a white spot or cavitated lesion over time. In order to develop effective mechanisms for remineralization of enamel, it is critical to understand the cycle of demineralization and remineralization, and how this balance can be influenced. While fluoride remains the gold standard in remineralization therapy, alternative medicaments are available — including nonfluoride and natural approaches (Figure 1).

Predominately an inorganic material, a tooth’s enamel layer is primarily composed of calcium hydroxyapatite crystals.3,4 These crystals are exceptionally large and strong, making them capable of withstanding the numerous forces placed upon them during daily function.4 Hydroxy­apatite, however, is prone to dissolving with repeated acid exposure, which can lead to caries lesion development when cariogenic bacteria are present.5 When hydroxyapatite is dissolved in response to acid exposure, calcium is removed from the tooth structure, leaving it demineralized (Figure 2 and Figure 3).3 Enamel begins to demineralize when the oral pH drops below 5.5, which can occur regularly, especially when patients have diets high in carbohydrates or are prone to snacking.6 However, the following systemic influences are critical in determining pH: acid reflux, xerostomia, frequent vomiting, a low baseline salivary pH, or medication usage. These factors can lead to chronically low intraoral pH; thus, demineralization will be prevalent. Therefore, while diet is a critically important controllable factor in the mineralization process, it is not the sole influencing factor.6

While the oral cavity is regularly in a state of demineralization, the body compensates for this. Human saliva is rich in calcium hydroxyapatite, which is absorbed by tooth structure to replace minerals lost during acid exposure. While this will not totally prevent the progression of demineralization, it can partially counteract the frequent drops in oral pH that accompany dietary and systemic complications. With constant demineralization of the enamel surface, eventually so many minerals are lost the enamel will be unable to remineralize based purely on the available calcium hydroxyapatite found in saliva. When the patient’s enamel deteriorates to this point, the clinician will need to implement an additional medicament to assist in the remineralization process.

FIGURE 1. In addition to fluoride therapies, clinicians have alternative, nonfluoride agents that can help promote remineralization. While additional evidence is needed to support efficacy, these include emerging management options, such as oral probiotics and bee propolis.
FIGURE 1. In addition to fluoride therapies, clinicians have alternative, nonfluoride agents that can help promote remineralization. While additional evidence is needed to support efficacy, these include emerging management options, such as oral probiotics and bee propolis.


Fluoride has remained the lifeline for dental professionals when treating demineralization. Fluoride works by influencing enamel integrity in three ways:

  1. Making the process of remineralization easier
  2. Making the process of demineralization more difficult
  3. Interfering with bacterial metabolism when found in high enough concentrations, thereby inhibiting the progression of a demineralized surface into a cavitated lesion7

When fluoride is readily available intraorally, calcium fluorapatite will be formed instead of calcium hydroxyapatite. Calcium fluorapatite is an altered crystal that does not dissolve as readily as calcium hydroxy­apatite. Whereas the critical pH of hydroxyapatite is 5.5, fluorapatite does not begin to demineralize until 4.5 — meaning, fluorapatite can withstand 10 times more acid exposure before demineralization will start to occur.8 As such, the availability of fluoride will make the tooth structure more resistant to demineralization over time, as calcium fluorapatite slowly replaces calcium hydroxyapatite.4

While fluoride has many benefits, it does have some clinical limitations, such as the inability to infiltrate cariogenic biofilms. An in vitro study found the application of fluoride in combination with L-arginine specifically addressed this particular concern, and the combination demonstrated superior enamel remineralization compared to fluoride alone.9 Additionally, L-arginine assisted in improving pH homeostatis, resulting in a decrease in demineralization frequency.9

Fluoride dentifrice may also be more effective at enhancing remineralization when tricalcium phosphate is added. This agent has been found to delay calcium from interacting with ionic fluoride, which allows more fluoride and calcium ions to be delivered to the demineralized enamel.10,11

Even though fluoride comes with decades of evidence-based support, some individuals are hesitant about fluoride exposure.12,13 Patient concerns range from fluorosis to a desire to follow holistic healthcare recommendations by rejecting the use of any toxin (which potentially includes fluoride).12,14–16 While patients’ rationales for why fluoride is systemically harmful are wide-ranging, fluoride hesitancy may lead to the refusal of fluoride for themselves and their children.13,14,17 As such, oral health professionals should be able and ready to discuss effective alternatives for treating demineralization.

FIGURE 2. A patient presenting with demineralization on teeth 7 through 10.
FIGURE 2. A patient presenting with demineralization on teeth 7 through 10.


Amorphous calcium phosphate (ACP) is a compound that contributes to remineralization of dental enamel. One drawback to ACP is its complicated delivery system necessitated by its unstable nature, making the reaction rate of calcium and phosphate difficult to control.18 A continued challenge for ACP use has been harnessing the benefit of calcium and phosphate before they are lost through precipitation.19 In an attempt to maximize the potential benefit of ACP and resolve the issue of complicated delivery, casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) has become popular.

As a milk protein casein derivative, CPP stabilizes calcium phosphate while also making it more available in dental biofilms.19,20 In clinical use, ACP is the component that actually remineralizes the enamel; CPP is added to help stabilize the ACP so that more is delivered to the enamel surface, rather than being lost through precipitation. Normally, calcium and phosphate are highly insoluble; however, in the presence of CPP, they will remain soluble and biologically available.19 The benefit of having this bioavailable calcium and phosphate includes buffering intraoral pH, which will reduce demineralization frequency.

Considered a reasonable alternative to fluoride, CPP-ACP demonstrates clinical efficacy in the reduction of white spot lesions, hypomineralization, mild fluorosis and dentinal hypersensitivity.18,19 Medica­ments containing CPP-ACP are available in either fluoride or fluoride-free formulations. For patients who are hesitant to use fluoride, non-fluoride CPP-ACP products represent an excellent alternative, with strong evidence supporting their effectiveness in remineralization.21 Patients who have a milk protein casein allergy should not use CPP-ACP, however.

Nano-hydroxyapatite is a biocompatible and bioactive material that has been studied as a remineralization agent. Featuring a similar structure to apatite crystals within enamel, nano-hydroxyapatite is well suited for remineralization, even in neutral pH conditions.18 Although promising, not enough long-term clinical trials have been conducted to support the efficacy of nano-hydroxyapatite as a comparable alternative to fluoride.

Calcium sodium phosphosilicate bioglass demonstrates promise in remineralization via the release of calcium and phosphate ions.18 It has primarily been used as a desensitizing agent, but research suggests it may enhance remineralization through apatite formation.22 More in vivo and clinical studies to support calcium sodium phosphosilicate efficacy are needed before it can be recommended as a fluoride alternative.18

FIGURE 3. Generalized demineralization and permanent white spot lesions.
FIGURE 3. Generalized demineralization and permanent white spot lesions.


Bee propolis, commonly referred to as “bee glue,” is a natural compound accumulated from different plants, buds and exudates.23–25 The primary use of propolis by bees is to repair and reinforce hives in order to prevent invaders from infiltrating or damaging them.23,24 While propolis samples vary slightly in composition, the primary components are resin, wax, essential oils, pollen, and other various organic compounds.24 For decades, bee propolis has been promoted as having a wide range of health benefits. Randomized controlled trials have demonstrated that propolis may have anticancer, antimicrobial, antifungal and anti-inflammatory effects, with possible oral health benefits — specifically, enamel remineralization.25

Recent studies have shown the application of propolis to demineralized enamel increased the microhardness of the enamel’s surface.25,26 Further, propolis application has demonstrated effectiveness at reducing the speed of enamel demineralization.26 It appears that propolis works by making calcium and phosphate more likely to be absorbed into the tooth structure, thus enhancing remineralization.26 Patients who are allergic to bee venom should avoid the use of propolis, as it may cause an allergic reaction. Propolis has demonstrated promise and continued research will help determine its effectiveness in remineralization therapy.

The use of oral probiotics is an emerging therapy for remineralization. Evidence is starting to demonstrate the oral microbiome is directly related to that of the gut; this further supports the oral-systemic connection.27,28 Probiotics’ specific mechanism of action in the remineralization process is not fully understood, which leaves ambiguity regarding their effectiveness.28 Research is limited, and the available evidence is contradictory. While probiotics do not directly remineralize enamel, some research shows they can assist the process by influencing oral pH, thereby increasing the likelihood that remineralization will take place by using available minerals within the saliva (Table 1).27,29

Numerous studies have demonstrated that oral probiotic administration using various vehicles resulted in a statistically significant reduction in cariogenic bacteria.28–30 An investigation by Stensson et al30 showed a reduction in caries when pregnant women were given an oral administration of Lactobacillus reuteri during the last month of their pregnancy, as well as to their infants during the first year of life.

However, there is concern that with regular or excessive use these bacteria could theoretically form their own biofilm, which could result in an increase in oral acid production, negating any beneficial effects.28 In response, researchers are working to develop a genetically modified bacterium that could change how oral probiotics operate in the oral cavity.31

Another issue with oral probiotic use is that researchers have been ineffective in permanently altering the oral microbiome; meaning, daily consumption of probiotics would be necessary in order to illicit a profound change in the oral bacteria.28 The need for such regular application could result in patient compliance issues, unless they were implemented into an application vehicle, such as dentifrice. Due to the lack of sufficient evidence from randomized controlled trials, a clinical recommendation cannot be clearly established for the use of probiotics for remineralization purposes.TABLE 1. Probiotics Effective at Reducing Caries Lesion Development


The use of fluoride has been heavily researched for decades, so it is challenging to develop a comparable alternative that comes with the same level of evidence-based research supporting clinical use for remineralization. When treating patients who are hesitant to use fluoride, clinicians should be ready to offer acceptable alternatives to assist in the remineralization process. In terms of nonfluoride therapy, CPP-ACP has strong evidence supporting its efficacy, as research has repeatedly shown clear improvements in remineralization and reduction in white spot lesions, along with other oral health benefits.21

When considering natural alternatives, bee propolis shows promise, although insufficient clinical studies are available to support using this agent in vivo. Similarly, oral probiotics have emerging research to support their use, but much of the mechanism of action has only been theorized and repeated exposure appears necessary to ensure an ongoing therapeutic effect.

Dental providers should strive to keep up with new research on these alternatives approaches to remineralization, as well-informed clinicians are best able to provide individualized education and recommendations that will support patients’ oral health.


  1. Abou Neel EA, Aljabo A, Strange A, et al. Demineralization-remineralization dynamics in teeth and bone. Int J Nanomedicine. 2016;11:4743–4763.
  2. Kawasaki K, Buchanan AV, Weiss KM. Biomineralization in humans: making the hard choices in life. Annu Rev Genet. 2009;43:119–142.
  3. ten Cate JM, Featherstone JD. Mechanistic aspects of the interactions between fluoride and dental enamel. Crit Rev Oral Biol Med. 1991;2:283–296.
  4. Pajor K, Pajchel L, Kolmas J. Hydroxyapatite and fluorapatite in conservative dentistry and oral implantology — a review. Materials (Basel). 2019;12:2683.
  5. Namestnikova IV, Rumyantsev VA, Egorova EN. [Influence of nutrition nature on acid-base balance in the mouth and the risk of dental diseases]. Eksp Klin Gastroenterol. 2016;5:12–15.
  6. Dawes C. What is the critical pH and why does a tooth dissolve in acid? J Can Dent Assoc. 2003;69:722–724.
  7. Moreno EC, Zahradnik RT. Demineralization and remineralization of dental enamel. J Dent Res. 1979;58:896–903.
  8. Wang LJ, Tang R, Bonstein T, Bush P, Nancollas GH. Enamel demineralization in primary and permanent teeth. J Dent Res. 2006;85:359–363.
  9. Bijle MN, Abdalla MM, Ashraf U, Ekambaram M, Yiu CKY. Enamel remineralization potential of arginine-fluoride varnish in a multi-species bacterial pH-cycling model. J Dent. 2021;104:103528.
  10. Rirattanapong P, Vongsavan K, Saengsirinavin C, Waidee S. Enhancing remineralization of primary enamel lesions with fluoride dentifrice containing tricalcium phosphate. Southeast Asian J Trop Med Public Health. 2017;48:494–500.
  11. Vanichvatana S, Auychai P. Efficacy of two calcium phosphate pastes on the remineralization of artificial caries: a randomized controlled double-blind in situ study. Int J Oral Sci. 2013;5:224–228.
  12. Guth S, Hüser S, Roth A, et al. Toxicity of fluoride: critical evaluation of evidence for human developmental neurotoxicity in epidemiological studies, animal experiments and in vitro analyses. Arch Toxicol. 2020;94:1375–1415.
  13. Leung E, Kerr D, Askelson N, Chi DL. Understanding topical fluoride hesitancy and refusal behaviors through the extended parallel process model and health belief model. J Public Health Dent. March 14, 2022. Epub ahead of print.
  14. Aoun A, Darwiche F, Al Hayek S, Doumit J. The fluoride debate: the pros and cons of fluoridation. Prev Nutr Food Sci. 2018;23:171–180.
  15. Strunecka A, Strunecky O. Mechanisms of fluoride toxicity: from enzymes to underlying integrative networks. Appl Sci. 2020;10:7100.
  16. Luke J. Fluoride deposition in the aged human pineal gland. Caries Res. 2001;35:125–128.
  17. Chi DL. Parent refusal of topical fluoride for their children: clinical strategies and future research priorities to improve evidence-based pediatric dental practice. Dent Clin North Am. 2017;61:607–617.
  18. Grohe B, Mittler S. Advanced non-fluoride approaches to dental enamel remineralization: the next level in enamel repair management. Biomater Biosyst. 2021;4:100029.
  19. Reema SD, Lahiri PK, Roy SS. Review of casein phosphopeptides-amorphous calcium phosphate. Chin J Dent Res. 2014;17:7–14.
  20. Gupta N, Marya CM, Nagpal R, Oberoi SS, Dhingra C. A review of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) and enamel remineralization. Compend Contin Educ Dent. 2016;37:36–39, quiz 40.
  21. Mehta R, Nandlal B, Prashanth S. Comparative evaluation of remineralization potential of casein phosphopeptide-amorphous calcium phosphate and casein phosphopeptide-amorphous calcium phosphate fluoride on artificial enamel white spot lesion: an in vitro light fluorescence study. Indian J Dent Res. 2013;24:681–689.
  22. Mony S, Rao A, Shenoy R, Suprabha BS. Comparative evaluation of the remineralizing efficacy of calcium sodium phosphosilicate agent and fluoride based on quantitative and qualitative analysis. J Indian Soc Pedod Prev Dent. 2015;33:291–295.
  23. Wagh VD. Propolis: a wonder bees product and its pharmacological potentials. Adv Pharmacol Sci. 2013;2013:308249.
  24. Pasupuleti VR, Sammugam L, Ramesh N, Gan SH. Honey, propolis, and royal jelly: a comprehensive review of their biological actions and health benefits. Oxid Med Cell Longev. 2017;2017:1259510.
  25. Khurshid Z, Naseem M, Zafar MS, Najeeb S, Zohaib S. Propolis: a natural biomaterial for dental and oral healthcare. J Dent Res Dent Clin Dent Prospects. 2017;11:265–274.
  26. Ali S, Farooq I, Bugshan A, Siddiqui IA, Al-Khalifa KS, Al-Hariri M. Efficacy of propolis in demineralizing artificially induced demineralization of human enamel — an in-vitro study. J Taibah Univ Med Sci. 2020;16:283–287.
  27. Chugh P, Dutt R, Sharma A, Bhagat N, Dhar MS. A critical appraisal of the effects of probiotics on oral health. J Function Foods. 2020;70:103985.
  28. Cagetti MG, Mastroberardino S, Milia E, Cocco F, Lingström P, Campus G. The use of probiotic strains in caries prevention: a systematic review. Nutrients. 2013;5:2530–2550.
  29. Sivamaruthi BS, Kesika P, Chaiyasut C. A review of the role of probiotic supplementation in dental caries. Probiotics Antimicrob Proteins. 2020;12:1300–1309.
  30. Stensson M, Koch G, Coric S, et al. Oral administration of Lactobacillus reuteri during the first year of life reduces caries prevalence in the primary dentition at 9 years of age. Caries Res. 2014;48:111–117.
  31. Haukioja A. Probiotics and oral health. Eur J Dent. 2010;4:348–355.

From Decisions in Dentistry. December 2022;8(12)40-43.

Leave A Reply

Your email address will not be published.

This site uses Akismet to reduce spam. Learn how your comment data is processed.

This website uses cookies to improve your experience. We'll assume you're ok with this, but you can opt-out if you wish. Accept Read More

Privacy & Cookies Policy